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yhdA [2019-06-26 09:07:56]
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yhdA [2019-06-26 09:07:56]

similar to NADPH-dependent azobenzene FMN reductase
locus
BSU09340
pI
6.51
mw
18.76 kDa
protein length
174 aa Sequence Blast
gene length
525 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.11|Resistance against toxic metals]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

    • Gene

    Coordinates
    1,010,445 1,010,969

    The protein

    Protein family

  • azoreductase type 2 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|CAFAE305B1771DD89E81F75A6713161393BE99A0|PgcM]:

    • [SW|Cofactors]

  • FMN [Pubmed|16752898]

    • Structure

  • [PDB|3GFQ] (99% identity),
  • [PDB|1NNI]

    • Expression and Regulation

    Operons

    genes
    [gene|8D570AEE5D78A9A3CDBB1F457630BAF5DB59BCFD|yhcY]-[gene|DD799ED3EB79457C27ED30C7B4DBBC2C8F962191|yhcZ]-[gene|345EC248C0ADF48B0C25B175241AAE982E0E9B78|yhdA]
    description
    [Pubmed|16816187]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16816187], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR]: activation, [Pubmed|16816187], in [regulon|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|LiaR regulon]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A689 (yhdA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/689 NBRP B. subtilis, Japan]
  • BKE09340 ([gene|345EC248C0ADF48B0C25B175241AAE982E0E9B78|yhdA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTGCCATTTATGACTAACA, downstream forward: _UP4_GGCGTCTAAACGCCGGGATT
  • BKK09340 ([gene|345EC248C0ADF48B0C25B175241AAE982E0E9B78|yhdA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTGCCATTTATGACTAACA, downstream forward: _UP4_GGCGTCTAAACGCCGGGATT

    • References

  • 16816187,16752898,20639339,20640807